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Histone H3 methylation patterns in Brassica nigra, Brassica juncea, and Brassica carinata species

Agnieszka Braszewska-Zalewska, Alina Dziurlikowska, Jolanta Maluszynska

University of Silesia, Faculty of Biology and Environmental Protection, Department of Plant Anatomy and Cytology, Jagiellońska 28, 40-032 Katowice, Poland.

Corresponding author: Agnieszka Braszewska-Zalewska (e-mail: ).


Paper handled by associate editor G. Jenkins

Published on the web 23 December 2011.

Genome, 2012, 55:(1) 68-74, 10.1139/g11-076

Abstract

Core histones are subjected to various post-translational modifications, and one of them, most intensively studied in plants, is the methylation of histone H3. In the majority of analyzed plant species, dimethylation of H3 at lysine 9 (H3K9me2) is detected in heterochromatin domains, whereas methylation of H3 at lysine 4 (H3K4me2) is detected in euchromatin domains. The distribution of H3K9me2 in the interphase nucleus seems to be correlated with genome size, chromatin organization, but also with tissue specificity. In this paper, we present the analysis of the pattern and level of histone H3 methylation for two allotetraploid and one diploid Brassica species. We have found that the pattern of H3K9me2 in interphase nuclei from root meristematic tissue is comparable within the analyzed species and includes both heterochromatin and euchromatin, but the level of modification differs not only among species but even among nuclei in the same phase of the cell cycle within one species. Moreover, the differences in the level of H3K9me2 are not directly coupled with DNA content in the nuclei and are probably tissue specific.

Keywords: Brassica, histone H3 methylation, image cytometry

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