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In vitro degradation of the moss Hylocomium splendens by three pleosporalean fungi

Melissa J. Day, Randolph S. Currah

Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada.

Corresponding author: Melissa J. Day (e-mail: ).

Published on the web 4 May 2011.

Canadian Journal of Microbiology, 2011, 57:(5) 382-391, 10.1139/w11-024

Abstract

Three darkly pigmented species of conidial fungi of the family Pleosporaceae isolated from plants colonizing the Saskatchewan Glacier forefield were examined for potential roles in the degradation of moss gametophytes. Curvularia inaequalis and Ulocladium atrum isolated from bryophytes Ditrichum flexicaule and Tortella tortuosa, respectively, and Chalastospora gossypii from Saxifraga oppositifolia were inoculated onto autoclaved gametophytes of the moss Hylocomium splendens. All three species of fungi caused mass losses of the moss gametophytes. In vitro enzymatic tests revealed that all three fungi degraded cellulose, while none degraded insoluble polyphenols. When this material was examined by scanning electron microscopy, it was evident that the fungi had eroded the outer wall layer of the moss leaf cells to some extent but not the inner layer containing more lignin-like compounds. Once the outer wall layer was removed, the cells easily disarticulated. It is proposed that accumulations of these phenolics-rich leaf fragments subsequently ameliorate the rooting environment for vascular plants and have the potential to support the growth of basidiomycetes and other fungi, potentially mycorrhizal with pioneer vascular plants.

Key words: decomposition, Pleosporaceae, Hylocomium splendens

References

  • Addy HD, Piercey MM, Currah RS. 2005. Microfungal endophytes of roots. Can. J. Bot. 83(1): 1-13 Abstract.
  • Azmi OR, Seppelt RD. 1998. The broad-scale distribution of microfungi in the Windmill Islands region, continental Antarctica. Polar Biol. 19(2): 92-100 CrossRef, ISI.
  • Bissett J, Parkinson D. 1979. Distribution of fungi in some alpine soils. Can. J. Bot. 57(15): 1609-1629 a Abstract.
  • Bissett J, Parkinson D. 1979. Fungal community structures in some alpine soils. Can. J. Bot. 57(15): 1630-1641 b Abstract.
  • Bissett J, Parkinson D. 1979. Functional relationships between soil fungi and environment in alpine tundra. Can. J. Bot. 57(15): 1642-1659 c Abstract.
  • Blanchette RA. 1995. Degradation of the ligninocellulose complex in wood. Can. J. Bot. 73: 999-1010 Abstract.
  • Butler MJ, Day AW. 1998. Fungal melanins: a review. Can. J. Microbiol. 44(12): 1115-1136 Abstract, ISI.
  • Caesar-TonThat TC. 2002. Soil binding properties of mucilage produced by a basidiomycete fungus in a model system. Mycol. Res. 106(8): 930-937 CrossRef, ISI.
  • Caesar-TonThat TC, Cochran VL. 2000. Soil aggregate stabilization by a saprophytic lignin-decomposing basidiomycete fungus I. Microbiological aspects. Biol. Fertil. Soils 32(5): 374-380 CrossRef, ISI.
  • Chapin FS, Walker LR, Fastie CL, Sharman LC. 1994. Mechanisms of primary succession following deglaciation at Glacier Bay, Alaska. Ecol. Monogr. 64(2): 149-175 CrossRef, ISI.
  • Coyne, M.S., and Thompson, J.A. 2006. Fundamental soil science. Delmar Cengage Learning, Clifton Park, N.Y., USA.
  • Crews TE, Kurina LM, Vitousek PM. 2001. Organic matter and nitrogen accumulation and nitrogen fixation during early ecosystem development in Hawaii. Biogeochemistry 52(3): 259-279 CrossRef, ISI.
  • Davey ML, Currah RS. 2009. Atradidymella muscivora gen. et sp. nov. (Pleosporales) and its anamorph Phoma muscivora sp. nov.: a new pleomorphic pathogen of boreal bryophytes. Am. J. Bot. 96(7): 1281-1288 CrossRef, ISI.
  • Davey ML, Tsuneda A, Currah RS. 2010. Saprobic and parasitic interactions of Coniochaeta velutina with mosses. Botany 88(3): 258-265 Abstract.
  • Edelmann HG, Neinhuis C, Jarvis M, Evans B, Fischer E, Barthlott W. 1998. Ultrastructure and chemistry of the cell wall of the moss Rhacocarpus pupurascens (Rhacocarpaceae): a puzzling architecture among plants. Planta 206(2): 315-321 CrossRef, ISI.
  • Erickson M, Miksche GE. 1974. Occurrence of lignin polyphenols in some mosses and liverworts. Phytochemistry 13(10): 2295-2299 CrossRef, ISI.
  • Farmer VC, Morrison RI. 1964. Lignin in sphagnum and phragmites and in peats derived from these plants. Geochim. Cosmochim. Acta 28(10–11): 1537-1546 CrossRef, ISI.
  • Flanagan, P.W., and Scarborough, A.M. 1974. Physiological groups of decomposer fungi on tundra plant remains. In Soil organisms and decomposition in tundra. Edited by A.J. Holding, O.W. Heal, S.F. Mclean, Jr., and P.W. Flanagan. Tundra Biome Steering Committee, Stockholm, Sweden. pp. 159–181.
  • Freeman KR, Martin AP, Karki D, Lynch RC, Mitter MS, Meyer AF, . 2009. Evidence that chytrids dominate fungal communities in high-elevation soils. Proc. Natl. Acad. Sci. U.S.A. 106(43): 18 315 - 18 320 a CrossRef.
  • Freeman KR, Pescador MY, Reed SC, Costello EK, Robeson MS, Schmidt SK. 2009. Soil CO2 flux and photoautotrophic community composition in high-elevation, ‘barren’ soil. Environ. Microbiol. 11(3): 674-686 b CrossRef, Medline, ISI.
  • Hébant, C. 1977. The conducting tissues of bryophytes. J. Cramer, Germany.
  • Hutchison LJ. 1990. Studies on the systematics of ectomycorrhizal fungi in axenic culture II. The enzymatic degradation of selected carbon and nitrogen compounds. Can. J. Bot. 68(7): 1522-1530 Abstract.
  • Hutchison LJ, Barron GL. 1997. Parasitism of algae by lignicolous Basidiomycota and other fungi. Can. J. Bot. 75(6): 1006-1011 Abstract.
  • Ligrone R, Carafa A, Duckett JG, Renzaglia KS, Ruel K. 2008. Immunocytochemical detection of lignin-related epitopes in cell walls in bryophytes and the charalean alga Nitella. Plant Syst. Evol. 270(3–4): 257-272 CrossRef, ISI.
  • Marx DH. 1969. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology 59(2): 153-163 ISI.
  • Rice AV, Tsuneda A, Currah RS. 2006. In vitro decomposition of Sphagnum by some micro fungi resembles white rot of wood. FEMS Microbiol. Ecol. 56(3): 372-382 CrossRef, Medline, ISI.
  • Sarkar P, Bosneaga E, Auer M. 2009. Plant cell walls throughout evolution: towards a molecular understanding of their design principles. J. Exp. Bot. 60(13): 3615-3635 CrossRef, Medline, ISI.
  • Tsuneda A, Maekawa N, Ohira I, Furukawa I. 1991. Incipient decay of Quercus serrata sapwood by Lentinus edodes and its inhibition by an antagonistic hyphomycete, Leptodontidium elatius. Can. J. Bot. 69(12): 2797-2805 Abstract.
  • Tsuneda A, Thormann MN, Currah RS. 2001. Modes of cell-wall degradation of Sphagnum fuscum by Acremonium cf. curvulum and Oidiodendron maius. Can. J. Bot. 79(1): 93-100 Abstract.
  • Viereck LA. 1966. Plant succession and soil development on gravel outwash of the Muldrow Glacier, Alaska. Ecol. Monogr. 36(3): 181-199 CrossRef, ISI.